DNA Technology Tree Map

DNA-Technology-Concept-Map
Explain rDNA
- Cutting DNA and pasting DNA from
different sources (also known as DNA
cloning)
- Involves several important tools
- Restriction enzymes
- Cloning vector
- DNA ligase
Cloning vs Gene Cloning
- Clone (Greek): described a cutting that is
used to copy a plant.
- Orchid
- Roseses
- Tapioca
- A state that all organisms share exact same
genetic material.
- Cloning
- Reproduction of organism as a whole
without sexual fertilization between male
and female gametes.
- Gene cloning
- Insertion of foreign DNA into another
organism.
What are required?
- Restriction endonuclease
- Also called DNA cutting enzyme or
restriction endonuclease (RE)
- The act of cutting called digestion
- Primarily in bacteria (as defense against
bacteriophage infection.
- Known as restriction site
- Other types of RE produce DNA fragments
with double stranded ends known as blunt
ends
- DNA ligase
- An enzyme responsible of joining two DNA
fragments
- Synthesize phosphodiester bond
- Two DNA fragments
- Between two compatible sticky ends or two
blunt ends.
- Common features between RE and DNA
ligase
- DNA cloning vector
- Pieces of DNA that can accept, carry and
replicate (clone) other pieces of DNA
- Important features
- Has the ability to replicate autonomously in
bacterial host
- Has a site for insertion of foreign DNA
- Has a selectable antibiotic resistant
marker(s)
- Ampicilin
- Kanamycin
- Tetracyclin
- DNA plasmid
- Screening of recombinants
- Usage of selectable markers available on
DNA plasmid
- Antibiotic resistant genes
- Lac Z gene (blue/white colony screening)
- Recombinant DNA tools
- Restriction endonuclease
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